A5713

Sigma

 

Monoclonal Anti-polyHistidine–Agarose antibody produced in mouse

clone HIS-1, purified immunoglobulin, PBS suspension, suitable for purification of HIS tagged recombinant proteins

Synonym:Monoclonal Anti-polyHistidine

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Description

Immunogenrecombinant polyHistidine tagged fusion protein.
Preparation NotePrepared using Protein A purified antibody coupled to cyanogen bromide-activated agarose at 2.0-2.4 mg antibody per mL resin volume.
Legal InformationThis Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.
SpecificityBinding capacity is at least 5 nmoles of HIS tagged protein per 1 ml settled resin (i.e., 25-250 μg protein/ml settled resin, for proteins of 5-50 kDa, respectively).
 The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.
Physical formSuspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Properties

antibody formpurified immunoglobulin
cloneHIS-1 (monoclonal)
formPBS suspension
application(s)immunoprecipitation: 5 using 6xHis tagged protein
isotypeIgG2a
suitabilitysuitable for purification of HIS tagged recombinant proteins
shipped inwet ice
storage temp.2-8°C

Safety

WGK Germany3